Innovation – Open view page
General information
| Innovation ID | 624 |
| Version ID | 479 |
| Innovation Title | Development of genome-editing tool for yam in Sub-Saharan Africa |
| Innovation description | CRISPR/Cas9-based genome-editing system and validation of its efficacy by targeting the phytoene desaturase gene (DrPDS) in a West African farmer-preferred Dioscorea rotundata accession Amola. |
| Reporting Staff | Leena Tripathi (IITA) |
| Year (Reporting) | 2020 |
| Reporting status | Approved |
| Innovation Type | Biophysical Research |
Stage reached
| Stage of Innovation | Stage 1: discovery/proof of concept |
| Year (Stage) | 2020 |
| Stage Description | The genome-editing efficiency in yam accession Amola was 83.3% (5 mutant events out of 6 transgenic events). These results demonstrated that the CRISPR/Cas9 could induce site-specific disruption of the PDS gene and produced stable phenotypical changes in yam. |
| Has a lead organization | yes |
| Lead Organization | International Institute of Tropical Agriculture |
| Top 5 contributors | University of Nairobi; Donald Danforth Plant Science Center; University of Missouri; Iowa State University |
Contributions and mapping
| All partners | International Institute of Tropical Agriculture-CGIAR Center/Program (Center); University of Nairobi-Academic Institutions (universities, colleges, etc.) |
| Main CRP | CGIAR Research Program on Roots, Tubers and Bananas |
| Flagship project | FP1-Discovery research for enhanced utilization of RTB genetic resources |
| Cluster | DI1.3-Genetically engineered RTB varieties with game-changing traits |
| Other CRPs-Flagships-Clusters |
Scope
| Geographic scope | National |
| Regions | |
| Countries | Nigeria,Benin,Togo,Ghana,Côte d'Ivoire,Kenya |
Targeted outcomes
| Main Sub-IDO | Enhanced genetic gain |
| Other Sub-IDO | |
| Other Sub-IDO |
Evidences
| Evidences | "Easter Syombua, Zhengzhi Zhang, Jaindra Tripathi, Valentine Ntui, Minjeong Kang, George Obiero, Edward Nguu, Kan Wang, Bing Yang, Leena Tripathi. (22/11/2020). A CRISPR/Cas9-based genome-editing system for yam (Dioscorea spp. ). Plant Biotechnology Journal, 19 (4), pp. 645-647." |
Linked Elements
| Milestones |
| Outcome Impact Case |
| Policy |