Innovation – Open view page
General information
| Innovation ID | 592 |
| Version ID | 457 |
| Innovation Title | Rapid diagnostic for Flavobacterium columnare developed. This bacteria is a causative agent of columnaris, a serious disease affecting numerous freshwater fish species worldwide. |
| Innovation description | Although several molecular protocols have been established to detect this pathogen, there still was a need to develop simpler on-field applicable techniques. Hence, we have developed a more practical and efficient assay to detect F. columnare based on the combination of recombinase polymerase amplification (RPA) and a lateral flow dipstick (LFD). Cross-reactivity against other bacteria and false results on 20 clinical samples were not observed, indicating its extremely high specificity and accuracy. |
| Reporting Staff | Vishnumurthy Mohan Chadag (WF) |
| Year (Reporting) | 2020 |
| Reporting status | Approved |
| Innovation Type | Research and Communication Methodologies and Tools |
Stage reached
| Stage of Innovation | Stage 1: discovery/proof of concept |
| Year (Stage) | 2020 |
| Stage Description | The robust performance of the assay to crude tissue samples and clinical specimens reflect its potential use in field application/low-resource settings. The use of a fast and affordable DNA extraction kit is imperative as part of our assay to make the detection of F. columnare more feasible. |
| Has a lead organization | yes |
| Lead Organization | Chulalongkorn University |
| Top 5 contributors | WorldFish; National Science and Technology Development Agency, National Center for Genetic Engineering and Biotechnology; Suez Canal University |
Contributions and mapping
| All partners | Chulalongkorn University-Academic Institutions (universities, colleges, etc.); National Science and Technology Development Agency-National Agricultural Research System (NARS); WorldFish-CGIAR Center/Program (Center) |
| Main CRP | CGIAR Research Program on Fish Agri-Food Systems |
| Flagship project | FP1-Sustainable aquaculture |
| Cluster | FP1-2-Fish health, nutrition and feeds |
| Other CRPs-Flagships-Clusters |
Scope
| Geographic scope | Global |
| Regions | |
| Countries |
Targeted outcomes
| Main Sub-IDO | Reduced livestock and fish disease risks associated with intensification and climate change |
| Other Sub-IDO | Reduced production risk |
| Other Sub-IDO | Closed yield gaps through improved agronomic and animal husbandry practices |
Evidences
| Evidences | "Mabrok, M. et al. (2020). Rapid visualization in the specific detection of Flavobacterium columnare, a causative agent of freshwater columnaris using a novel recombinase polymerase amplification (RPA) combined with lateral flow dipstick (LFD) assay. Aquaculture, 531: 735780." |
Linked Elements
| Milestones |
| Outcome Impact Case |
| Policy |