Innovation – Open view page
General information
| Innovation ID | 591 |
| Version ID | 456 |
| Innovation Title | Genetic diversity of tilapia lake virus genome segment 1 from 2011 to 2019 described and a newly validated semi-nested RT-PCR method developed |
| Innovation description | The newly established PCR protocol is 100 times more sensitive than previous protocols when comparatively assayed with RNA extracted from infected fish. The assay was also shown to be specific when tested against negative control samples. |
| Reporting Staff | Vishnumurthy Mohan Chadag (WF) |
| Year (Reporting) | 2020 |
| Reporting status | Approved |
| Innovation Type | Research and Communication Methodologies and Tools |
Stage reached
| Stage of Innovation | Stage 1: discovery/proof of concept |
| Year (Stage) | 2020 |
| Stage Description | Validation experiment with RNA extracted from naturally infected fish specimens collected in 2013–2019 yielded positive results for all samples tested, confirming that the newly designed primers and detection protocol against TiLV segment 1 are an alternative option for TiLV diagnosis in laboratories where conventional RT-PCR method is preferred. |
| Has a lead organization | yes |
| Lead Organization | Mahidol University, Faculty of science, Center of Excellence for Shrimp Molecular Biology and Biotechnology |
| Top 5 contributors | WorldFish; National Science and Technology Development Agency, National Center for Genetic Engineering and Biotechnology |
Contributions and mapping
| All partners | Mahidol University, Faculty of science, Center of Excellence for Shrimp Molecular Biology and Biotechnology-Academic Institutions (universities, colleges, etc.); Suan Sunandha Rajabhat University-Academic Institutions (universities, colleges, etc.); WorldFish-CGIAR Center/Program (Center) |
| Main CRP | CGIAR Research Program on Fish Agri-Food Systems |
| Flagship project | FP1-Sustainable aquaculture |
| Cluster | FP1-2-Fish health, nutrition and feeds |
| Other CRPs-Flagships-Clusters |
Scope
| Geographic scope | Global |
| Regions | |
| Countries |
Targeted outcomes
| Main Sub-IDO | Reduced livestock and fish disease risks associated with intensification and climate change |
| Other Sub-IDO | Reduced production risk |
| Other Sub-IDO | Increased capacity for innovation in partner research organizations |
Evidences
| Evidences | Taengphu, S. et al. (2020). Genetic diversity of tilapia lake virus genome segment 1 from 2011 to 2019 and a newly validated semi-nested RT-PCR method. Aquaculture, 526: 735423. [https://hdl.handle.net/20.500.12348/4178] |
Linked Elements
| Milestones |
| Outcome Impact Case |
| Policy |